Antioxidant and Antidiabetic Activity of Decalepis Hamiltonii Leaves Extract

  • Ashok Kumar Faculty of Pharmacy, Bhagwant University, Ajmer, Rajasthan, India 305004
  • Sattwik Das Faculty of Pharmacy, Bhagwant University, Ajmer, Rajasthan, India 305004
  • Neeraj Sharma Faculty of Pharmacy, Bhagwant University, Ajmer, Rajasthan, India 305004

Abstract

In most nations, diabetes mellitus is a serious and rising health issue. The goal of the study was to compare the antidiabetic effects of leaves extracts of Decalepis hamiltonii in normal and diabetic rats induced with alloxan. Using 120 mg/kg of alloxan monohydrate, diabetes was induced. In order to considerably lower blood glucose levels in both normal and alloxan-induced diabetic rats, D. hamiltonii methanolic extract was given at doses of 200 mg and 400 mg, along with glibenclamide at a dose of 7 mg/kg bwt. Additionally, the injection of the extract dramatically reduced the levels of AST, ALT, triglycerides, and total blood cholesterol while simultaneously raising the amount of liver glycogen. In order to perform the oral glucose tolerance test (OGTT), separate groups were given doses of D. hamiltonii methanolic extract (200 mg, 400 mg), and glibenclamide (7.5 mg), which effectively reduced blood sugar levels following an oral glucose load at all time points. These findings indicate that diabetic rats' blood glucose levels were successfully lowered by the methanolic extract of D. hamiltonii leaves. The antioxidant potential of the leaves was measured using various in vitro assays. The methanolic extracts showed high antioxidant activity measured as scavenging of DPPH, superoxide and hydroxyl radicals. The antioxidant activity did not correlate with the phenolic content of the extracts. These results demonstrate the antioxidant potency of the leaves extracts which could be the basis for its alleged health promoting potential of D. hamiltonii. The leaves of D. hamiltonii could serve as a new source of natural antioxidants or nutraceuticals with potential applications to reducing the level of oxidative stress and related health benefits.

Keywords: Decalepis hamiltonii, Antidiabetic, Antioxidant, Alloxan, AST, OGTT

References

1. Manickam D, Periyasamy L. Antidiabetic effect of methanolic extract of Decalepis hamiltonii root (wight and Arn) in normal and alloxan induced diabetic rats. Journal of pharmacy research. 2013 Jan 1;6(1):166-72.
2. Matsui T, Tanaka T, Tamura S, Toshima A, Tamaya K, Miyata Y, Tanaka K, Matsumoto K. α-Glucosidase inhibitory profile of catechins and theaflavins. Journal of agricultural and food chemistry. 2007 Jan 10;55(1):99-105.
3. Scheen AJ. Drug treatment of non-insulin-dependent diabetes mellitus in the 1990s: achievements and future developments. Drugs. 1997 Sep;54(3):355-68.
4. Engelgau MM, Narayan KV, Saaddine JB, Vinicor F. Addressing the burden of diabetes in the 21st century: better care and primary prevention. Journal of the American Society of Nephrology. 2003 Jul 1;14(suppl 2): S88-91.
5. Piedrola G, Novo E, Escobar F, Garcia-Robles R. White blood cell count and insulin resistance in patients with coronary artery disease. InAnnales D'endocrinologie 2001 Feb 1 (Vol. 62, No. 1 Pt 1, pp. 7-10).
6. Yaryura‐Tobias JA, Pinto A, Neziroglu F. Anorexia nervosa, diabetes mellitus, brain atrophy, and fatty liver. International Journal of Eating Disorders. 2001 Nov;30(3):350-3.
7. Bhattaram VA, Graefe U, Kohlert C, Veit M, Derendorf H. Pharmacokinetics and bioavailability of herbal medicinal products. Phytomedicine. 2002 Jan 1; 9:1-33.
8. Herson PS, Ashford ML. Activation of a novel non-selective cation channel by alloxan and H2O2 in the rat insulin-secreting cell line CRI-G1. The Journal of physiology. 1997 May 5;501(Pt 1):59.
9. Anup S. Antioxidant Properties and Chemopreventive Potential of the Bioactive Constituents Of the Roots Of Decalepis Hamiltonii (Doctoral dissertation, University of Mysore).
10. Anita BS, Okokon JE, Okon PA. Hypoglycemic activity of aqueous leaf extract of Persea americana Mill. Indian J Pharmacol. 2005; 37:525-6.
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How to Cite
Ashok Kumar, Sattwik Das, and Neeraj Sharma. “Antioxidant and Antidiabetic Activity of Decalepis Hamiltonii Leaves Extract”. Current Research in Pharmaceutical Sciences, Vol. 13, no. 2, July 2023, pp. 93-101, doi:10.24092/CRPS.2023.130202.
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Research Articles